We offer immunoregulation theory that explained links between normal immune parameters and risk of unfavorable condition development. Our theory finds experimental support in these results. Immune accentuations create unfavorable background for embryo implantation and pregnancy development. We show that multiple immune accentuations significant predictive for implantation and pregnancy failure after IVF. We showed physiology nature of immune accentuations association and unfavorable phenotype formation. Similar diagnostic issue can be also in other useful for prognosis in immune-dependent clinical conditions.
Presenter: Boris V. Dons’koi He has completed his PhD at the age of 31 years from institute of Pediatrics, Obstetrics and Gynecology, National Academy of Medical Sciences of Ukraine, Kiev, Ukraine. He presently is a Senior Research Fellow in laboratory of immunology of institute of Pediatrics, Obstetrics and Gynecology, National Academy of Medical Sciences of Ukraine. Research interests: immunology of reproduction, immunology methods and immunodiagnostic models. He has published more than 30 papers in reputed journals and more than 80 abstracts in scientific congresses.
Bacillus strains from rhizosphere are effectively used in agriculture for plant growth promotion and plant protection against phytopathogens. Despite a long history of application of biopesticides in agriculture, it is still unclear what the difference is between selected biologically active strains and multiple less active isolates from plant rhizosphere. In the current work, twelve bacterial strains obtained from the Ukrainian Collection of Microorganisms were fully sequenced and their activities were tested in multiple bioassays on various in vivo and in vitro models. The strains were selected in a way to represent biologically active organisms and their less active counterparts from the same species. Comparative genomics revealed sixteen genes characteristic for the most active biotechnological strains but absent in the less active organisms independently on species belonging. Among these genes, there were oxidative stress and antibiotic resistance genes, sugar permeases, transcriptional regulators and endonucleases. Involvement of the horizontal gene transfer was supposed. A new technique was developed for genetic barcoding of the strains of industrial importance to allow identification of species, subspecies, clades and individual strains in the environment by using metagenomic approaches. Validation of the innovative barcoding techniques on publically available metagenomic datasets demonstrated preferences in distribution among different ecological niches between different sub-species of the studied Bacillus organisms. This analysis will be helpful to identify biotechnological strains to be the most appropriate for a specific crop or soil type.
Presenter: Oleg N. Reva completed his PhD in 1995 at the age of 25 years from the DK Zabolotny Institute of Microbiology and Virology in Kyiv, Ukraine. He did his postdoctoral study on bioinformatics in 2002-2004 in the Medical High School of Hanover, Germany. Currently he is an associated professor on bioinformatics in the Centre for Bioinformatics and Computational Biology at the University of Pretoria, South Africa. Research interests: bacterial genomics, genetic barcoding and molecular docking in biotechnology and medicine. He published 77 papers and 6 book chapters cited around 1500 times, H-index 20.
This oral presentation is devoted to the research of an anti-endotoxin immunity and polymorphism of genes receptors of CD14 (C-159T) and TLR-4 (Asp299Gly) in patients with different subtypes of asthma. The endotoxin-dependent chronic inflammation in asthma is accompanied by increased concentrations of serum anti-endotoxin antibodies of class M, G; higher level of sCD14 in induced sputum and decreased concentrations of secretory anti-endotoxin immunoglobulin A. The presence of genotype CT + TT (C159T-CD14) has reduced the risk of asthma in non-atopic, neutrophilic, refractory subtype, but has increased risk in eosinophilic subtype and in patients with infrequent exacerbations. The presence of genotype AG + GG (Asp299Gly-TLR-4) has increased the risk of asthma in atopic, eosinophilic, corticosteroid-sensitive sybtypes and in patients with early onset asthma, reversible obstruction. It was proved that efficacy of inhaled corticosteroids and leukotriene modifiers in asthma depends on Asp299Gly polymorphism of TLR-4.
Presenter: Yuriy Bisyuk MD, Ph.D., Associate-Professor of Clinical Immunology and Allergy with a section of Medical Genetics of the Bogomolets National Medical University. Member of the Ukrainian Society of Clinical Immunology and Allergy, the European Academy of Allergy and Clinical Immunology, the American College of Allergy, Asthma and Immunology, American Academy of Allergy, Asthma and Immunology. He has clinical specialization in Allergology, Clinical Immunology and Pulmonology. He passed international fellowship in immunology at the Immunology Research Institute of New England, Boston, USA in 2009. Research interests: investigation of genetic polymorphism of chronic inflammation in asthma. Author has more than 70 articles and 2 monographs. He was awarded honorary diplomas of the Ministry of Health of Ukraine and the Council of Ministers of the Autonomous Republic of Crimea.
Cell preparation "Cryopreserved human umbilical cord blood" was administered in patients with heart failure (HF). This preparation contained stem cell (SC) population isolated from human umbilical cord blood (CB) and conserved at a temperature of -196° C. We determined the level of IL-2, IL-6 and IL-10 in the serum of patients before and 1, 3, 6, 9 months after the CB SC transplantation (CB SCT). IL-2 is a growth factor for T cells and can also enhance the production of B-cells from CD34 +/- CB mononuclear cells. We showed no changes in IL-2 serum level in the postransplant period that corresponded with the IL-2 levels of healthy individuals. We also showed the absence of changes in the levels of anti-inflammatory IL-10 during whole postransplant period, what probably point on the absence of disturbances in Th-2-mediated immune response. In contrast, we revealed elevated serum levels of pro-inflammatory cytokine IL-6 in patients prior to CB SCT, which indicates on activation of Th-1 inflammatory response and participation of IL-6 in the pathogenesis of HF. On 6 month of follow-up after CB SCT serum level of IL-6 significantly decreased and reached normal values of healthy individuals; however on 9 month level of IL-6 increased, indicating activation of Th-1 immune link and the ability of this cytokine to inhibit Th-2 immune response.
Presenter: Alina A. Stasenko Dr.Sc., she has completed her PhD at the age of 33 years, defended doctoral thesis at the age of 49 years at Taras Shevchenko Nashional University of Kiev. Currently she is a Senior Researcher on immunology in the Laboratory of Immunology at the A.A.Shalimov National Institute of Surgery and Transplantation in Kyiv, Ukraine. Research interests: humoral and cellular factors of local and systemic immunity in patients with purulent diseases and purulent complications of surgical interventions. She has published 104 scientific papers.
In the lecture current concepts concerning the main functional phenotypes of mono- and polymorphonuclear phagocytes are systematized, molecular mechanisms of their formation are considered, and the functional polarization concept of phagocytes is critically analyzed. Phagocyte functional polarization in disease pathogenesis is reviewed. In addition, diagnostic and prognostic significance of evaluating functional polarization phagocytes of different populations and different location is discussed.
Presenter: Larysa M. Skivka ScD., PhD. She has completed her PhD at the age of 28 years from R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine and postdoctoral studies from Taras Shevchenko National University of Kyiv. She presently is a head of the Department of Microbiology and General Immunology of SCE “Institute of Biology” of Taras Shevchenko National University of Kyiv. Research interests: immunomodulation as a component of adjuvant therapy under the tumor growth, functional polarization of phagocytes in the pathogenesis of inflammatory diseases. She is an author more than 100 scientific publications. She has published more than 23 papers in reputed journals and more than 60 abstracts in scientific congresses.
Presenter: Volodymyr Ivanytsia Sc.D., Professor, Honored Worker of Science of Ukraine, vice Rector, Head of Department of Microbiology and Virology of Odessa I.I.Mechnikov National University, vice President of the Association of Microbiologists of Ukraine, the chief editor of the scientific journal "Microbiology and Biotechnology". He organized and held the International Summer School of Molecular Microbiology and Biotechnology for young scientists on the basis of ONU and Zabolotny Institute of Microbiology and Virology NASU. Research interests: marine microbiology, biological diversity of microorganisms, acquisition of resistance of bacteria in vivo under the influence of toxins, bacteriocins, bacteriophages, mechanism of interaction between bacteria and plant, biotechnology of plant protection and the environment. He developed the concept of variability of microorganisms in the direction of their aggressive growth properties under conditions of chronic pollution of the environment by chemical toxicants, made a significant contribution to the study of microbial biodiversity of the Black Sea. Author of more than 170 articles, 7 books and dictionaries, 6 textbooks, coauthor of 25 environment protection documents.
The development of colonic inflammation in colitis is associated with increase of the blood-brain barrier permeability and the loss of central dopaminergic neurons. From the other hand, the disturbance of central dopaminergic neurons during Parkinson’s disease (PD) is characterized by colonic constipation. Constipation might be associated with the growth of pathogenic microflora as a result of that the disruption of intestinal barrier integrity, inflammation development and further exacerbation of PD symptoms. We will talk on our recent data whether the colonic inflammation is primary associated with the early stages of PD pathogenesis or PD increases the susceptibility to colonic inflammation development.
Presenter: Ganna Tolstanova has completed her PhD in Human and Animal Physiology (2003) and Doctor of Science degree in Biochemistry (2011) at Taras Shevchenko National University of Kyiv, Kyiv, Ukraine. From 2006 to 2013, has worked as Visiting Research Fellow in VA Medical Center, Long Beach; the David Geffen School of Medicine, UCLA, Los Angeles, CA, USA. Currently she is Chief of Research Department, Taras Shevchenko National University of Kyiv, Kyiv, Ukraine. She is the ordinary member of Physiological Society (www.physoc.org) and member of Editorial boards of peer-reviewed journals: Frontiers in Pharmacology and Ukrainian Biochemical Journal. Research interests: role of endothelial and epithelial permeability, endothelium-associated transcription factors and growth factors in low GI tract pathogenesis. She published 50 papers and 4 book chapters, H-index 9.
The results of our research led to the holistic understanding of biogeochemical role of microscopic fungi and built the foundation of geomycology – the new area of research studying the fungal role in the fundamental geological processes including the metal cycling and mineral transformations in nature. As a part of elucidation of the mechanisms of fungal biogeochemical activity, we studied the nature of the speciation of toxic metals bioaccumulated in the process of minerals transformation by fungi using a synchrotron-based X-ray absorption spectroscopy. It was found that toxic metals in biomass are predominantly coordinated by oxygen ligands: phosphate and carboxylate, and the ligands nature depends on various physico-chemical and biological factors. We showed the spatial-temporal evolution of the biogeochemical transformations of copper in the colonies of micromycetes and their microenvironment.
Presenter: Marina Fomina has completed her PhD in microbiology at the age of 28 years old at DK Zabolotny Institute of Microbiology and Virology in Kyiv, Ukraine. Her first postdoctoral fellowship at the Biological Sciences Institute of Dundee University, Scotland, UK, started her long-term work and collaboration with world leading Geomicrobiology Group of Prof Geoffrey M. Gadd. She was awarded with Microscopy Awards from the British Mycological Society in Nottingham in 2004 and from Royal Microscopical Society in London in 2006. She is currently working as a senior scientific researcher at DK Zabolotny Institute of Microbiology and Virology in Kyiv, Ukraine. Research interests: microbial ecology, mycology, geomicrobiology, microscopy, biotechnology, bioremediation and recently micro-eukaryotic probiotics. She is an author of over 100 scientific publications. She published around 40 papers and 6 book chapters cited over 1200 times, H-index 19.
We have isolated from the water of Yavoriv Lake and studied microorganisms that perform the processes of sulfur compounds metabolism. It was shown that sulfate- and sulfur-reducing bacteria take part in the transformation of organic compounds, utilizing them as the donors of electrons for dissimilatory sulfate-, sulfur- and metals reduction. Hydrogen sulfide, produced by bacteria, reacts with metals ions, producing insoluble sulfides. In the processes of anaerobic respiration these bacteria can also reduce metals ions and transform them into less toxic forms. In the water, enriched by sulfates, the intensity of sulfate reduction determines the velocity of purple and green photosynthetic sulfur bacteria growth and is the catalyzer of elements cycles. Results of analysis of heavy metals ions contents in water have shown their rapid accumulation in bottom sediments that leads to the malfunction of microbiota functioning. Ions of metals with variable valence cause the production of active oxygen metabolites. Our research was also concerned to the effects of some metals to bacterial cells, the system of their anti-oxidative defense, which is important for the understanding of the ways of metabolism regulation in these microorganisms in the process of natural- and wastewaters purification.
Presenter: Svitlana Hnatush defended PhD thesis "Formation of Ethanol by Lactose-Utilizing Yeasts and its Role in the Regulation of Carbohydrates Metabolism Enzymes" in D. K. Zabolotnyi Institute of Microbiology and Virology NAS Ukraine. She is working in Ivan Franko National University of Lviv, having undergone a way from the engineer of the Department of Microbiology to the Dean of the Faculty of Biology. From 2014 she is a chief of the Department of Microbiology. In 2015 acquired professor's academic degree. Research interests: physiological and biochemical properties of microorganisms that support sulfur cycle in nature, effects of heavy metals compounds to bacterial cells, microbiota of rock dumps of coal mines. Co-author of 2 textbooks, 4 manuals, 2 monographs, 7 patents, 107 articles.
The new polycomponent bioformulations on the base of selected soil streptomycetes: Streptomyces avermitilis IMV Ас-5015 (Avercom, Avercom nova-2), S.netropsis IMV Ас-5025 (Phytovit) and S. violaceus IMV Ас-5027 (Violar) were elaborated. These bioformulations contain the biologically active metabolites of producers: antiparasitic antibiotics, free amino- and fatty acids, lipids, phytohormones (auxins, cytokinins, gibberellins and brassinosteroids). The new bioformulations increased plant resistance to damaging action of parasitic nematodes. The analysis of density of nematode population complex showed the diminishing of total amount of plant parasitic nematodes in plant roots that were treated by Avercom, Averсom nova-2, Violar and Phytovit by 4−10 times as compared to untreated control plants. The new microbial product cause reprogramming of plant genome, i.e. induce synthesis in plant cells of small regulatory si/miRNA with immune-protective against plant parasitic nematodes properties. As a result plant resistance to this pest is increased, the improving of the crop quality and yield increase are observed.
Presenter: Galyna Iutynska Corresponding member of NAS of Ukraine, Doctor of biology sciences Professor, Deputy Director on scientific work of D.K.Zabolotny Institute of Microbiology and Virology of the NAS of Ukraine. He graduated from I.I.Mechnikov Odessa University, department of biology. Research interests: cell biology, general and soil microbiology, functioning of microbial cells in natural ecosystems and in biotechnological processes, studying of structural and functional properties of soil microbiocoenosis, microbial synthesis of antibiotics and other biologically active compounds. She is an author of over 300 scientific publications including 13 monographs, textbook, 18 patents. She was awarded of D.K.Zabolotny Prize of NAS of Ukraine and Prize of Presidents of Academy of Sciences of Ukraine, Belarus and Moldova.
The study is devoted to investigation of probiotic properties of bacteria of Bacillus genus and interaction of the preparations on their base with macroorganism. Accurate taxonomic position of the strains Bacillus sp. UCM B-5139 and Bacillus sp. UCM B-5140 was determined. Their membership of the species B. amyloliquefaciens ssp. plantarum was substantiated. Antimicrobial characteristics of the strains UCM B-5139 and UCM B-5140 was examined. Ability of the strain UCM B-5140 to synthesize antibiotics surfactin, fengycin, bacillaene, macrolactin and difficidin, and also siderophore bacillibactin was established. It was demonstrated that the strains UCM B-5139 and UCM B-5140 of the bacteria B. amyloliquefaciens ssp. plantarum synthesize the complex of proteases, bacteriolytic and yeast-lytic enzymes, extracellular amino acids and exopolysugars. In the experiments in vivo the effect of veterinary preparation Endosporin on immune reactivity indices of macroorganisms was studied. Endosporin facilitates the increase of macrophages and lymphocytes activity, normalization of immune system status in animals with dysbacteriosis, it is also characterized by antitumor properties. Probiotics’ effect for medical practice of Biosporin and Subalin was investigated. In the experiments in vivo the ability of the preparation Subalin to increase the effect of anti-tumor vaccine was demonstrated.
Presenter: Larysa Safronova Sc.D., until 2010 she worked as a senior researcher of Antibiotics research department, since 2010 - Head of Innovation and Technology Transfer department of DK Zabolotny Institute of Microbiology and Virology, NAS of Ukraine. Research interests: biological properties of bacteria of the genus Bacillus, development and introduction of probiotic preparations on the basis of live bacilli strains, effects of probiotic preparations on macroorganisms. Author of 100 scientific publications, section of the book (in English) and 8 patents.
Presenter: Andrii Kurchenko MD, PhD, DrMedSci, Professor, Head of Clinical Immunology and Allergology of Medical Genetics Section of the National Medical University He graduated with honors from Chernivtsy medical institute due to specialty medical care. In 2008 he defended the doctor's dissertation on topic "Atopic dermatitis: characteristics for changes in system and local immunity". Co-author of textbook and 3 study guides, 1 typical and 5 detailed curricula, author of 170 scientific works. He has the highest professional category in three clinical specialties: clinical immunology, allergology and dermatovenerology. Deputy chief editor in special journal "Immunology and allergology: science and practice". Full member in European and American academies of allergology and clinical immunology. In 2011 he was elected to the post of vice-president in Ukrainian society of specialists in immunology, allergology and immune rehabilitation. Since April 2012 he occupies the post at department of clinical immunology and allergology with section of medical genetics.
The degradation of metallic surfaces due to atmospheric corrosion is a well-known problem for many exposed steel structures. Bringing sea water into this equation causes an even more aggressive environment and an increased corrosion effect. Сorrosion is a major cause of marine structural failures which led to huge financial costs for maintaining. How do we factor in the presence and action of the biological actors in a ballast tank? What is the role of bacteria in ballast tank corrosion? And how should we use this for future strategies in the discovery of the real extent of biocorrosion in ship’s ballast tanks?
Presenter: Geert Potters
studied Biology at the University of Antwerp, Belgium, and completed his Ph.D. in Biology in 2002 there with a dissertation titled “The role of ascorbate in the regulation of cell cycle and cellular redox status in a Nicotiana tabacum L. cv. Bright Yellow-2 cell culture”. He worked in the same lab as National Science Foundation funded postdoc for three years and then transferred, in 2005, to the starting department of Bioscience Engineering, where he started a research line on bamboo and phytoremediation. Given the opportunity to join the staff at the Antwerp Maritime Academy, he took up the position of Professor in Chemistry and Ecology at the Academy in 2011. Dr. Potters is also the head of the scientific commission of the Botanical Garden of Merksplas, Flanders, and the President of the non-profit organization Bio-MENS, focusing on popularization of science towards adolescents.
He published 41 papers, 10 peer reviewed elaborate congress proceedings, one handbook on Marine Pollution, 33 popularising articles and 3 book chapters.
Short review of recent data on adaptive immune response in bacteria and archea related to functional activity of CRISPR/Cas systems. The major accent is made on the influence of bacterial/archeal CRISPR/Cas systems on the evolution of bacteriophages, as well as on the aspects of co-evolution of bacteria and their phages.
Presenter: Valerii Polishchuk
defended his doctoral thesis "Forecasting and regularities of plant viruses in biocenoses of Ukraine" in 2000, at the age of 42, at the Institute of Microbiology and Virology DK Zabolotny NASU in Kiev. Since 2002, he worked as a professor, and from 2004 to date – as the Head of the Department of Virology of the ESC "Institute of Biology" of Taras Shevchenko National University of Kyiv.
Valerii Polishchuk has authored and coauthored 8 manuscripts and more than 200 scientific papers, many of which have been published in renowned international journals. He has 5 patents for inventions. Valerii Polishchuk has raised 12 PhDs and 1 Doctor of Sciences (Scientific school "The structure and function of viruses in different environmental conditions"). Now he supervises 5 PhD and 1 Doctor of Sciences’ thesises.
In work it was supported free radical theory of aging and theory of aging by Mechnikov for changes in quantitative and qualitative composition of intestinal microflora in old age, which made it possible to offer periodic use multiprobiotic "Symbiter acidophilic" concentrated as a drug that prevents age-related changes in the antioxidant defense system in different environments of the body, in microbiocenosis of colon, secretion of hydrochloric acid in the stomach, transport of water, ions of sodium and chlorine through the epithelium of the colon. It was proved that multyprobiotic slows aging, shown to reduce concentrations in serum biomarker of aging - urea nitrogen and increase in life expectancy of rats by 11.9%.
Presenter: Beregova Tetyana Volodymyrivna awarded State Prize of Ukraine in Science and Technology, Honored Worker of Science and Technology of Ukraine, in 1985 defended his thesis, and in 2001 - his doctoral thesis, which was devoted to the central inhibition of gastric secretion. All her working life is related to the Taras Shevchenko National University of Kyiv. In 2005, Beregova T.V. awarded the academic rank - professor in the specialty human and animal physiology. The last 15 years she works in the post of Head of Laboratory "Pharmacology and Experimental Pathology" of Educational and Scientific Centre «Institute of Biology» of Taras Shevchenko National University of Kyiv. Research interests: the physiology, pathology, pharmacology of the gastrointestinal tract, the mechanisms of cell-tissue lesions and cytoprotection, the role of microflora in the digestive system in the maintenance of structural integrity and functional state of the digestive organs. She is the author of nearly 600 scientific papers, 3 manuals, 4 monographs, 15 patents.
Review of the key approaches to stimulate plant-microbe interactions, in particular biological nitrogen fixation. Biological nitrogen fixation is a powerful factor in the preservation and reproduction of soil fertility, increase productivity of agrocenoses. Considerable attention is paid to the study of the microflora of the soil under the sowing of the plants, which are the agents of the effect of external factors and indicators of ecosystem status and succession processes taking place in it. The widespread use of environmentally safe technologies using microbial preparations nitrogen-fixing microorganisms is an important prospect of obtaining high-quality competitive agricultural production, soil conservation and the environment.
Presenter: Patyka Volodymyr Pylypovych Doctor of Science, Professor. Academician of NAAS. Academician of Higher School AS of Ukraine. In 1975 he defended his PhD thesis dedicated to phytotoxic substances of soil microorganisms, in 1992 - his Dr.Sc.: "The role of nitrogen-fixing microorganisms in increasing of crops productivity". 1997-2000. - Director of the Institute of Agriculture Microbiology UAAS; in 2000-2005. - Director of the Institute of agroecology and biotechnology UAAS, from 2005 - Professor of Biotechnology department of Environmental Safety Faculty in National Agrarian University. Since 2006 - Head of pathogenic bacteria department, D.K. Zabolotny Institute of Microbiology and Virology NAS of Ukraine. He published over 540 scientific papers, several reviews, problem articles, 15 monographs, received 36 patents. The founder of "Agro-ecological Journal", member of the editorial board of "Microbiological Journal", "Microbiology and Biotechnology" and "Physiology and Biochemistry of Cultivated Plants", "Bulletin of Kharkiv National Agricultural University”.
The parenteral route of antigen delivery can induce in vaccinated subjects high levels of serum antibodies mainly of IgG class, which can protect organism from the systemic pathogen spread. In contrast, the oral route for vaccine delivery provides effective mucosal IgA-dependent immunity, that can protect body from bacterial colonization and also prevent the pathogens or toxins penetration into the blood. However, the efficacy of free antigens oral administration is limited by their degradation in the gastrointestinal tract and poor absorption by M-cells. This review describes recently developed carrier systems for the delivery of antigens to the mucosal immune system.
Presenter: Kolybo Denys graduated Taras Shevchenko National University, Biological Faculty, General Microbiology and Immunology department in 1995. In 1995-1998 he was PhD student at the Institute of Bioorganic and Oil Chemistry of the National Academy of Sciences of Ukraine where he defended his PhD thesis in 1999. He did his postdoctoral study on immunology in 1999-2003 at the Molecular immunology department at Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine. He got his habilitation (Doctor of Sciences) at the Institute of Molecular Biology and Genetics of the National Academy of Sciences of Ukraine in 2011. Current place of work is Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, where he has the position of Deputy Director on Scientific Affair. From 2000 till now he is also Associated Professor at the Department of Basic Microbiology and Immunology, Taras Shevchenko National University. Research interests: Immune recognition of bacterial antigens.
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